Applications of Polymerase Chain Reaction

Applications of PCR:

• PCR can be used to amplify a specific gene in different individuals of a species. These copies can be used for cloning.
• PCR can be used to study & identify the multiplication changes in the amplified (or) clones gene.
• PCR can be used to study “DNA polymorphism” in the genome using known sequences as primers.
• PCR can be used to identify the transgenic animals among the normal animals and detect the presence of a gene transferred into an organism (transgene).
• In another hybridization methods (Southern, Northern & Western) radioactive atoms will use for identify transgene.  But by using PCR no need to radioactive atoms(P³²) for transgene identification. This PCR detection takes in one day for completing this identification.
• Use to estimate the DNA frequencies in the species.
• PCR use to determine the physical location of genes in chromosomes.
• By using PCR, to determine the sex of embryos.

Principle and Procedure of PCR

See the Video Tutorial of the PCR

PCR Tutorial