1. Why proline and gylcine are exceptions in Ramachandran plot?
2. Let’s assume Hexokinase having Vmax 100 mmol/min with having Km 10 mM for Glucose.
a. With [S] = 100 mM, which will increase the velocity more; a 10 fold decrease in Km or a 10 fold increase in Vmax
b. With [S] = 10 mM, which will increase the velocity more; a 10 fold decrease in Km or a 10 fold increase in Vmax
3. A population contains 1800 individuals, 162 with yellow fruit and 1638 with green fruit. You know that fruit color segregates as a single Mendelian locus with two alleles. An experimental cross between a green-fruited and yellow-fruited individual yields only green-fruited offspring. Assuming that the population is in Hardy-Weinberg equilibrium, how many of the green-fruited individuals are homozygous at this locus?
4. Describe a strategy to sequence a 4 kb fragment of DNA.
5. Why is proline considered as a ‘compatible solute’ in plants? Mention the two molecules from which its biosynthetic pathways originate.
6. How the Pyruvate dehydrogenase (PDH), a key enzyme in citric acid cycle regulated? Name one metabolite involved in activation and one in deactivation of PDH, and mention their respective targets.
7. Show pictorially E. coli lac operon. What kind of regulation occurs in lac operon. Why?
8. a. What is popular plasma membrane model. Why is it so.
b. What are the four major phospholipids present in the plasma membrane of mammalian cells?
9. How different Cycloheximide and chloramphenicol in inhibiting protein synthesis ?
10. If you are given the genotypic frequencies of AABB, AaBB, aaBB, AABb, AaBb, aaBb, AAbb and Aabb in a mating experiment. How will you test whether the loci A and B are linked
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